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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)



Genomes and Gene Technologies


A genome of an organism is the entire genetic information found within an organism. Genes found in the
genome code for polypeptides, this is refer to as coding DNA of which only 1.5%…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)





Electrophoresis is a method of separating DNA fragment in order of their size with the accuracy of one base
length. The technique involves a:

Gel plate containing agarose (sugar), covered in buffer solution.
Electrodes attached at both…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)





Automated DNA sequencer can sequence the DNA in a similar fashion to PCR, where:

The primer anneals to the 3 end base of the template strand
DNA polymerase extends the primer by adding
free nucleotides
However polymerase…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)





Recombinant DNA is a section of DNA formed when by joining DNA sections from two different sources. It is
carried out through:
A DNA Probe (50 to 80 nucleotides long strand) locates the DNA sections required as…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)

A "heat shock" is given, where temperatures in the culture are lowered to 0oC and quickly raised to
40oC (increases the rate of plasmid intake; however the efficiency is still only 0.25%)
Conjugation, where bacteria's exchange copies…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)





Engineering case studies (insulin and golden rice)


Bacteria can be genetically engineered, using the techniques
studied to produce insulin. This can be done through:

Extracting mRNA that codes for insulin from pancreatic
tissues (islet of Langerhans), using…

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Sameer Jahabarali Biology

Unit 5 Module 2- Biotechnology and Gene Technologies (part 2)

Enzymes already present in the endosperm convert carotenoid in to -carotene



Xenotransplantation is the process of transplanting organs or tissues between animals of different species
(allotransplantation- same species). This is done by genetically engineering animals so that…

Comments

sara

do you have your notes for reponding to the environment?

angela

hey type in responding to the environment there's a word document available that is really good

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