Microbiology lecture 8 0.0 / 5 ? BiologyThe MicrobiomeUniversityNone Created by: willmiddlehamCreated on: 03-04-15 16:47 What is the microflora? The microbes found within/on a given region 1 of 13 What is the microbiota? All microorganisms that live in a defined region 2 of 13 What is the microbiome? The collective genome of a given microbiota 3 of 13 What do you use to classify bacteria? 16S/18S rRNA sequences 4 of 13 How do you classify bacteria? Isolate the DNA --> PCR 16S gene --> Sequence PCR product --> Analyse data 5 of 13 Why use 16S rRNA to classify bacteria? It is common to all bacteria. Core gene. 6 of 13 What is it called when a bacterium is able to invade a sterile site form a non-sterile site? Infection 7 of 13 What are the main bacteria that occupy the skin? Gram +ves dominant 8 of 13 What are the main bacteria that occupy the gut? Bacteroidetes 9 of 13 How does the microflora help in the digestive tract? Process complex dietary carbohydrates 10 of 13 What do bacteroidetes produce? How are they important? Sphingosine/ sphingolipids (important in signal transmission and cell recognition in mammals) 11 of 13 What do they help with? (in the human body) Digest complex sugars 12 of 13 What are the bi-products of polysaccharide metabolism done by bacteroidetes? and how is this useful? Short chain fatty acids aka. volatile fatty acids - major source of energy for us and protect colon from cancer 13 of 13
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