Microbiology

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  • Microbiology 1-2
    • Micro-organisms
      • Recycle Nutrients
      • Bioremediation (Breakdown Pollutants)
      • Immune Development and Human Health
      • Food Production
      • Disease
      • First observed under a microscope in 1665
    • Biogenesis
      • Prevention of food spoilage
      • understand causes of disease
      • prevention of infections
    • Pioneers
      • Pasteur
        • Fermentation and pasteurisation
        • Germ Theory
        • Vaccines and Hand Washing
      • Koch
        • Organism present in disease
        • must be grown in culture
        • Pure culture of organism must cause disease
        • must be reisolated from animal
    • Bacteria
      • Cytoplasm
        • Thick, Semi transparent and elastic
        • 80% water
        • Contains all molecules required for life
          • Nucleoid (DNA)
          • Ribosomes, site of protein synthesis, comprised of RNA
      • Cell Membrane
        • Selective carrier proteins
          • semi selective barrier that regulates uptake of nutrients
        • Phospholipid bilayer
        • Encloses cytoplasm
      • Cell wall
        • Gram Negative
          • Endotoxin, lipopolysaccharrides
            • Evades phagocytosis
            • Barrier to antibiotics
          • Less peptidoglycan
        • Antigenic, common site of antibiotic action
        • Protect from rupturing (rigid)
          • Strength provided by mucoprotein peptidoglycan
        • Gram Positive
          • Much peptidoglycan
      • Glycocalyx
        • Capsules
          • Firmly attached
          • Highly organised
        • Slime Layer
          • unorganised
          • Loosely attached
        • sticky substance made of polysaccharrides/peptides or both
        • Biofilms/immune evasion
        • Protects from dehydration
    • Tree of Life
      • Archaea
        • Live in Extremes
        • High Temp
        • High Salt
        • Alkali/Acid
        • Digestive Tract
        • Petrol
  • Teichoic Acids
    • Gram Positive
      • Much peptidoglycan
  • Cell Membrane
    • Selective carrier proteins
      • semi selective barrier that regulates uptake of nutrients
    • Phospholipid bilayer
    • Encloses cytoplasm

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