Microbiology lecture 7

HideShow resource information
What are the two groups of nutrients bacterial cells need?
Macronutrients, micronutrients
1 of 10
In what amounts are macronutrients required?
C - 50%, O - 17%, N - 13%, P - 2.5%
2 of 10
How do you calculate the final cell number?
N = No(initial cell no.) x Z^n(no. of generations)
3 of 10
How do you measure bacterial growth by dilutions?
Colony forming units extrapolated to give cell numbers in original culture. Only measure viable cells. Underestimates for cells in chains or clusters
4 of 10
What is cytoplasmic pH?
pH 7
5 of 10
What process can cause pH to change often?
Batch, chemostat growth. So need a buffer (Na2HPO4 and KH2PO4)
6 of 10
Give properties of chemostats
1) System is open 2) Fresh media in 3) Spent media and cells out 4) Continuous 5) Can be kept in 1 state
7 of 10
Change in biomass..
dx/dt = ux - Dx
8 of 10
At a steady state dx/dt=0 therefore ....
D=u --> D=g
9 of 10
What is umax?
When D is too high bacteria cannot stay in culture. The fastest growth rate that bacteria can grow in that media.
10 of 10

Other cards in this set

Card 2

Front

In what amounts are macronutrients required?

Back

C - 50%, O - 17%, N - 13%, P - 2.5%

Card 3

Front

How do you calculate the final cell number?

Back

Preview of the front of card 3

Card 4

Front

How do you measure bacterial growth by dilutions?

Back

Preview of the front of card 4

Card 5

Front

What is cytoplasmic pH?

Back

Preview of the front of card 5
View more cards

Comments

No comments have yet been made

Similar Biology resources:

See all Biology resources »See all How do we cultivate bacteria? resources »