microbiology lecture 8

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Pili and fimbriae are Fine hair like filaments on the surface of many Gram negative bacteria. what are they NOT involved in?
motility
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Fimbriae composed from what protein?
20 kDa
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what are longer pili or fimbriae?
pili
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Both involved in attachment of bacteria to host cells
: select incorrect statement
tool utilised in motility
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Bacteria within a human host are constantly losing and reforming fimbriae, they are fragile structures, that aid in evasion of immune system: give an example of what can change the antigenic structure of their pili / fimbriae
Bacteria, e.g. N. gonorrhoea
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what allows sex pilis to be formed and allow bacterial conjugation. F+ and F- (F+ indicates fertility)
plasmids
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After conjugation the previously negative cell has a copy of the plasmid and is able to pass it on to what
another cell
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Sex pili and conjugation is an Efficient manner for transfer of what
antimicrobial resistance
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what of E. coli can also mobilise the chromosome to be transferred through cell to cell contact
F plasmid
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what integrates into the chromosome and leads to transfer of large regions of the host genetic material and extensive genetic recombination.
episome
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Hfr strain stands for what?
high frequency recombination
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what cells are poor recipients for the same or similar plasmid
Cells already containing a plasmid
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SEX PILI AND CONJUGATION STEP 3: ONE STRAND OF PLASMID DNA TRANSFERS TO THE RECIPIENT, WHAT HAPPENS NEXT?
recipient synthesises a complementary strand to become a F+ cell, the donor synthesises a complementary strand restoring its complete plasmid
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what do capsules and slime layers consist of
polysaccharide or sometimes protein
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genereal term for Consist of polysaccharide or sometimes protein is?
glycocalyx
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what is a Thick viscous gel outside and attached to cell wall
Capsule
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what is Capsule loosely attached to cell wall and easily washed off
Slime
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both slime and capsules are hydrophilic, they Appear as what around cells in a Gram stain
halos
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capsules and slime layers have variable thicknesses, what is Too thin to be seen on light microscope, and detected by antisera?
Microcapsule of E. coli and Salmonella spp
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name 2 exceptions for capsules being immunogenic and stimulate host antibody response: select incorrect statement
Streptococcus pneumoniae- apsule contains N-acetylneuraminic acid which is found on host cells
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Advantages to bacteria: select incorrect answer
proliferation to mast cells
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Bacterial chromosomes are: Single circular chromosome of double stranded DNA, called a nucleoid

300 to 400 m in length and supercoiled like a rubber band

Genes arranged linearly along the chromosome

Size range from 600 to 9500kb (9.5Mb)
and are compo
Adenosine
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Prokaryotic protein synthesis takes place on which ribosome,es in the cytoplasm?
70S (30S+50S)
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30S contains how many RNA (small subunit)
16S
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50S contains how many RNA (large subunit)
23S
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Bacterial mRNA is polycistronic, meaning what?
multiple genes on one mRNA
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during Prokaryotic protein synthesis, Protein translation is via
tRNA and mRNA
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during Prokaryotic protein synthesis, Location of protein encoding genes determined by looking for Open Reading Frames (ORF): select incorrect statement
Central codon
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Cytoplasm – carbon storage polymers: have an Amorphous gel containing:select incorrect statement
Variety of mineral granules for proliferation of mast cells
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Cytoplasm – carbon storage polymers: One of the most common inclusion bodies consists of?
poly-B-hydroxybutyric acid (PHB)
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in cytoplasm, as well as PHB, what is also used as storage product?
glycogen
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describe glycogen in cytoplasm
Starch-like polymer of glucose subunits
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Other cards in this set

Card 2

Front

Fimbriae composed from what protein?

Back

20 kDa

Card 3

Front

what are longer pili or fimbriae?

Back

Preview of the front of card 3

Card 4

Front

Both involved in attachment of bacteria to host cells
: select incorrect statement

Back

Preview of the front of card 4

Card 5

Front

Bacteria within a human host are constantly losing and reforming fimbriae, they are fragile structures, that aid in evasion of immune system: give an example of what can change the antigenic structure of their pili / fimbriae

Back

Preview of the front of card 5
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