Cell Fractionation 0.0 / 5 ? BiologyCellular processes and structureASAQA Created by: chickpeatweetCreated on: 09-11-14 16:49 Why must the sample tissue be in a cold solution? To reduce enzyme activity 1 of 11 Why must the sample tissue be in a buffered solution? To maintain a constant pH 2 of 11 Why must the sample tissue be in an isotonic solution? To prevent the shrinking/bursting of organelle due to gain/loss of water 3 of 11 What does homogenation do? Break the cells to release the organelle 4 of 11 What is the fluid produced by homogenation called? Homogenate 5 of 11 Why is the fluid produced by homogenation filtered? The homogenate is filtered to remove complete cells and other debris 6 of 11 What does the process of ultracentrifugation do? The the contents of the homogenate are separated 7 of 11 What types of organelle sink to the bottom when the sample is spun at a low speed? Heavy organelle such as nuclei 8 of 11 What types of organelle sink to the bottom when the sample is spun at a medium speed Organelle such as mitochondria 9 of 11 What types of organelle sink to the bottom when the sample is spun very fast? Light organelle such as lysosomes 10 of 11 Between each increase in speed, what is removed from the top of the solution? A fluid called supernatant 11 of 11
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