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What is cell fractionation?
It is the process where cells are broken up and the different organelles they contain are seperated out
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Before cell fractionation can begin why is the tissue placed in a cold, isotonic, buffered solution?
cold- To reduce enzyme activity Isotonic- To prevent organelles bursting or shrinking Buffered- To maintain a constant pH.
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What is Ultracentrifugation?
It is the process by which fragments in the filtered homogenate are seperated in a machine called an ultracentrifuge
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What are limitations of the transmission electron microscope?
1. The whole system must be in a vacuum and therfore living specimens cannot be observed 2. A complex staining process is required and even then the image is only in black and white. 3. The specimen must be extremely thin.
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What are the functions of the SER?
1. To synthesise, store and transport lipids 2. To synthesise, store and transport carbohydrates
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What are the functions of the RER?
1. To provide a large surfacearea for the synthesis of proteins and glycoproteins. 2. To provide a pathway for the transport of materials,especially proteins throughout the cell.
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Whatare the functions of the golgi apparatus?
1. Add carbohydrate to proteins to form glycoproteins. 2. Produce secretory enzymes, such as those secreted by the pancreas. 3. Secrete carbohydrates 4. Transport, modify and store lipids. 5. Form lysosomes
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What are the functions of lysosomes?
To break down material ingested by phagocetic cells, such as white blood cells. 2. relese enzymes to the outside of the cell in order to destroy material around the cell. 3. digest worn out organelles. 4. break down cells after they have died.
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Card 2

Front

Before cell fractionation can begin why is the tissue placed in a cold, isotonic, buffered solution?

Back

cold- To reduce enzyme activity Isotonic- To prevent organelles bursting or shrinking Buffered- To maintain a constant pH.

Card 3

Front

What is Ultracentrifugation?

Back

Preview of the front of card 3

Card 4

Front

What are limitations of the transmission electron microscope?

Back

Preview of the front of card 4

Card 5

Front

What are the functions of the SER?

Back

Preview of the front of card 5
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