Cell fractionation is the process where cells are broken up and the different organelles they contain are seperated out. Before cell fractionation the tissue is placed in a cold, isotonic, buffered solution. The solution is
Cold- To reduce enzyme activity that might break down the organelles
Isotonic- To prevent organelles from bursting or shrinking as a result of the osmotic gain or loss of water
Buffered- To maintain a constant PH.
Two stages to cell fractionation:
Cells are broken up by a homogeniser which releases the organelles fromt the…