Cell Fractionation - AQA

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Cell fractionation is the process where cells are broken up and the different organelles are separated out.

The tissue is placed in a cold, isotonic buffered solution.

Cold - Reduces enzyme activity that could otherwise break down the organelles.

Isotonic - Prevents organelles from bursting or shrinking as a result of osmotic gain or loss of water because the water potential is the same as the cell.

 Buffered - Maintains a constant pH.

There are two stages to cell…



which exam board is this for,could you please tag it to that exam boards,thanks.

Good work!  :D

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