Cell Fractionation Unit 1!

Unit 1

Cell Fractionation

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Cell Fractionation
Sediment: Matter that settles at the bottom of a liquid
Supernatant: The liquid above a solid residue
Homogeniser: Breaks cells up and releases organelles
Ultracentrifuge: The process by which the homogenate is separated in a machine called a
Cell Fractionation: A process in which cells are broken down and their organelles separated
Cold, Isotonic Buffer Solution
Cold - Denatures enzymes so they do not attack organelles
Isotonic - To balance the movement of water in and out of the cell via osmosis
Buffer - To maintain a constant pH
Order of Sediments...
Debris > Nucleus > Mitochondria


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