Cell Fractionation

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Why must the sample tissue be in a cold solution?
To reduce enzyme activity
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Why must the sample tissue be in a buffered solution?
To maintain a constant pH
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Why must the sample tissue be in an isotonic solution?
To prevent the shrinking/bursting of organelle due to gain/loss of water
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What does homogenation do?
Break the cells to release the organelle
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What is the fluid produced by homogenation called?
Homogenate
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Why is the fluid produced by homogenation filtered?
The homogenate is filtered to remove complete cells and other debris
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What does the process of ultracentrifugation do?
The the contents of the homogenate are separated
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What types of organelle sink to the bottom when the sample is spun at a low speed?
Heavy organelle such as nuclei
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What types of organelle sink to the bottom when the sample is spun at a medium speed
Organelle such as mitochondria
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What types of organelle sink to the bottom when the sample is spun very fast?
Light organelle such as lysosomes
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Between each increase in speed, what is removed from the top of the solution?
A fluid called supernatant
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Card 2

Front

Why must the sample tissue be in a buffered solution?

Back

To maintain a constant pH

Card 3

Front

Why must the sample tissue be in an isotonic solution?

Back

Preview of the front of card 3

Card 4

Front

What does homogenation do?

Back

Preview of the front of card 4

Card 5

Front

What is the fluid produced by homogenation called?

Back

Preview of the front of card 5
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