Calibration 0.0 / 5 ? BiologycalibrationOtherNone Created by: une-meduseCreated on: 29-12-14 16:49 Where the relationship is established between a signal/measurement/response, and the amount/conc of analyte Calibration 1 of 30 Calibration needs to be used to Validate a method 2 of 30 To ensure equipment is working accurately and correctly, what is useful Determine the calibration 3 of 30 Who performs calibrations? Lab scientists, manufacturers, service engineers 4 of 30 ________ are used to ensure more accurate quantification in chromatographic techniques Internal standards 5 of 30 Internal standards help to correct for systematic and _____ errors within methods, such as pipetting, injection, recovery and extracrion efficiency Random 6 of 30 For mass spec, the internal standard is usually deuterium, however this is Expensive 7 of 30 Defined by the PHE as all the planned and systematic activities put in place to ensure quality requirements for a product or service to be fulfilled Quality assurance 8 of 30 This includes: audits, record keeping, training, instrument calibration, transport, storage, sampling, supplies and raw materials Quality assurance 9 of 30 Quality assurance aims to __________ Understand errors 10 of 30 Major sources of error can be found in the _________, instrument and methods used Operator 11 of 30 _______ errors, aka determinate errors can be constant or proportional, and can be measured and corrected Systematic 12 of 30 _______ errors, aka indeterminate errors can be minimised or managed, but can not be eliminated Random 13 of 30 Systematic errors Determinate errors 14 of 30 Random errors Indeterminate errors 15 of 30 Bias, precision and LoD are part of Method validation 16 of 30 The difference between an observed/measured mean and a reference value Bias 17 of 30 Measure of trueness, arises due to systematic error Bias 18 of 30 You need sufficient ________ to detect practically significant bias Precision 19 of 30 A suitable and representative _____ of analyte levels and sample matrices should be chosen appropriate to the method Range 20 of 30 _______ of a method is the closeness in agreement of multiple independant measurements, which have been obtained under stipulated conditions Precision 21 of 30 ______ is a measure of the spread of results, can be concentration dependant, can be matrix dependant Precision 22 of 30 ____________ arises from random error and effects Precision 23 of 30 Precision - Repeatability Replicate measurements, one lab, one analyst, same equip, short time scale 24 of 30 Precisiion - Intermediate precision Replicate measurements, one lab, diff analyst, diff day, diff equip 25 of 30 Precicion - Reproducibility Replicate measurements, diff lab, diff analysts, diff equi 26 of 30 _________ represents a combination of bias and precision Accuracy 27 of 30 Shewhard chart and Cusum chart are different types of Quality control charts 28 of 30 The minimum amount of analyte that can be detected reliably Lod 29 of 30 The minimum amount of analyte that can be quantified with acceptable precision and uncertainty Loq 30 of 30
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