2 Major Activities- Strand Separation, DNA synthesis
Strand Separation- Unwinding proteins/Helicase unwinds DNA, Helix destabilising proteins (allows replication and prevents recoiling)
Gyrase- cause break in DNA strand which coils it back on itself allows access for replication
DNA Synthesis- Polymerase activity, only add base in 5' to 3' direction one base at a time, primer essential
Primer- region of double strandedness, RNA Polymerase = primase
Discontinuos synthesis (Lagging Strand)- antiparrallel strands, one strand starts and stops, OKAZAKI FRAGMENTS produced, primers have to be removed, DNA Polymerase 3 fills in the gaps. At least 1 strand is discontinuous!
DNA Ligase covalently bonds OKAZAKI Fragments and fill primer spaces!
Comments
No comments have yet been made