cell fractionation

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  • Created by: Angelique
  • Created on: 17-12-12 10:09

cell fractionation

1) cut tissue

  • cold to slow reactions
  • isotonic to stop osmosis
  • buffer to stop ph changes

2) grind tissue

3) filter to remove insoluble tissue

4) centrifugue

  • low speed - nuclei
  • medium speed - mitochondria / chloroplasts
  • high speed - er / golgi
  • very high speed - ribosomes

5) organelle free cytoplasm is left over

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