DNA Fingerprinting 0.0 / 5 ? BiologyDNA and inheritanceGCSEOCR Created by: meganrelseyCreated on: 16-06-16 20:23 What is the first stage in DNA fingerprinting collect a DNA sample 1 of 13 What is the second stage in DNA fingerprinting Fragment DNA with restriction enzymes 2 of 13 What is the third stage in DNA fingerprinting "load" fragments into a gel, run an electric current through the gel. DNA is negitive and will move to the positive end 3 of 13 What is the fourth stage in DNA fingerprinting smaller fragments move faster creating a band pattern on the gel, then you read the pattern 4 of 13 whats the first step in engineering bacteria to produce insulin identify the gene which controls the production of insulin 5 of 13 Whats step 2 remove the DNA from the cell by cutting it out with a restriction enzyme. DNA is left with sticky ends 6 of 13 Whats step 3 a loop of bacterial DNA (a plasmid) is cut open with the same restriction enzyme so it has sticky ends to 7 of 13 what is step 4 the insulin is inserted in the plasmid, the sticky ends allow another enzyme (ligase) to join DNA strands together 8 of 13 what are the four stages of infectious disease 1) micro-orgaism enters the body 2) reproduces rapidly 3) toxins are produced 4) toxins cause symptoms 9 of 13 whats the first stage in viruses reproducing virus attaches itself to a specific host cell where it injects its genetic material 10 of 13 whats stage 2 virus then uses the host cell to make the components of a new virus 11 of 13 whats stage 3 the host cell splits open releasing the new virus 12 of 13 how do bacteria reproduce binary fission 13 of 13
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