Temperature/pH: Temperature monitor and control to maintain the optimum conditions, water jackets remove excess heat due to respiration, commercial and sophisticated monitors improve the control of temperature and pH, add acid/alkali/buffer to maintain optimum pH.
Culture/ medium: Pure culture is needed for pure product (only one microorganism), vessel and medium should be sterilised and mixing of the culture to improve contact with nutrients.
Aeration/mixing: Sterile air produced by a filter, not needed by bacteria/fungi if anaerobic, in commercial machines air inlets use spargers and other devices to improve aeration, forced aeration may be needed for maximum growth of aerobes, mixing can be improved by a seperate mixer, provide oxygen for aerobes, improves contact with nutrients and prevent sedimentation (bacteria/fungi sink to the bottom)
Product: Batch fermentation - nutrients added at the start and nothing added in the fermentation processs, products harvested at the end. Continuous fermentation - nutrients and products are harvested throughout the process.
- the vessel should be sterilised beforehand and an appropriate sterile medium used. During use the vessel openings must be protected from contamination by filters, aseptic conditions and handiling are required to maintain purity.
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