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Microbiology Tracking Test Revision

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Bacteria Classification
By shape: The shape is due to rigid cell wall. Round (coccus) Rod shaped (bacillus)
Spiral shaped (spirillum) Curved rod (vibrio)
By gram staining: Gram negative: The additional lipopolysaccharide layer doesn't allow
the absorption of
crystal violet down to the thin peptidoglycan layer, therefore stains red by counterstain…

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Culturing In Laboratories

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Aseptic Technique To prevent contamination into the culture To prevent contamination to
the environment by the culture.
Preventing contamination: Equipment and media are sterilised before use, often by heat.
Autoclave is used at 121°C for 15 minutes. Inoculating loop is sterilised by passing over
Bunsen flame. Plastics which can't be…


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