OCR AS Biology Unit 2 (F212) revision notes on enzymes to complete with presentation

This word document is notes on enzymes for people studying A level as biology unit 2 (F212). It matches up to a presentation that i have already put on here and as you go throught the presentation you can fill in the gaps. Or, if you're feeling adventuros you can do it without, please yourself. 

This resourse is for students studying AS OCR biology unit 2 (F212) 
(I'm only saying this a billion times becuase i always find it annying when people dont put what exam board it is so yay no guess work)

Thank you and good luck with revision.  

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  • Created by: Faye
  • Created on: 15-04-13 20:13
Preview of OCR AS Biology Unit 2 (F212) revision notes on enzymes to complete with presentation

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What is an enzyme?
They are ............................................... (have unique
shapes)
They act as a catalyst
They have active sites which fit specific
................................
They are affected by PH and temperature
What affects the activities of an enzyme?
PH
A PH that is too .................. for an enzyme causes the H+ ions to attack the tertiary
structure of the enzyme which causes them to ..............................
A PH too ............... for an enzyme does not give the enzyme enough H+ ions to
maintain the tertiary structure
When a PH is at its optimum for an enzyme the reaction occurs at its maximum rate.
The optimum PH changes form enzyme to enzyme.
Eg/ Pepsin and Trypsin
Rate
of
Reaction
PH value
The different optimum values are due to the places where the enzymes are found.
Pepsin is found in the stomach so therefore
needs a low PH optimum so it doesn't
denature in the stomach acid.
Trypsin is found in the small intestine which
has alkaline conditions and therefore the
optimum PH needs to be high.
Temperature

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Page 2

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Optimum temperature gives the maximum rate of reaction producing the maximum of
product.
Enzyme concentration
Increasing the enzyme concentration increases the rate of reaction because there
are more ................................
The limiting factor for enzyme concentration is the .........................................
because the substrates get used up.
Rate of
reaction
Substrate concentration
Increasing the concentration of the substrate .......................... the chance of
collisions and therefore increases the ...............................................
The limiting factor of substrate concentration is .......................................…read more

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Rate of
reaction
concentration of substrate
Inhibitors
Competitive ­ They have a similar shape to the
substrate and bind to the active site preventing the
............................ from binding and therefore
decreasing the rate of reaction.
Noncompetitive This attaches to the enzyme, away from the active site causing the
shape of the active site to change and therefore the ...................... can no longer
......................... to the active site and therefore the enzyme cannot catalyse the
reaction.…read more

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Metabolism
Used to control enzyme reactions
The last product is an competitive inhibitor of the first enzyme
The uses of enzymes…read more

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Natural
Chemical reactions
Digestion
Protection ­white blood cells ­ lysins
Commercial
Detergents
Food and drink
Advantages
Specific ­ no by products
Re usable
Less energy, hear or pressure required
Questions:
1) Define what a catalyst is.
2) State the 4 factors that can affect the activities of enzymes.
3) What is the difference between a competitive and a non competitive
Inhibitor?
4) Explain why the end product normally inhibits the first enzyme.…read more

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Draw a graph to show the effect of increasing the substrate concentration for a fixed
enzyme concentration.…read more

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