Practical 6
- Created by: Former Member
- Created on: 26-04-19 12:49
View mindmap
- Practical 6
- Aseptic techniques
- Washing hands and disinfecting/sterilising surfaces to kill any pathogens
- Flame the sterilising inoculating loop
- Flaming the neck of the culture tube
- Lifting the petri dish a small amount to prevent contamination
- Streaking the plate quickly with the inoculating loop
- Aseptic techniques are needed to avoid contamination which could affect results and potentially harm someone
- Water can be used as a control as it will not kill any bacteria, showing any bacteria that is killed is due to the antibiotic
- Inhabitation zones
- = where bacteria cannot grow due to the antibiotic
- The larger the zone, the more bacteria killed
- If there is no zone, that means the bacteria is resistant to the antibiotic, as it was able to grow
- Bacteria can become resistant to an antibiotic due to a random mutation, resulting in a new allele being formed
- The resistant bacteria survive, and the non-resistant bacteria are killed off, resulting in a strain of bacteria resistant to a certain antibiotic
- Bacteria can become resistant to an antibiotic due to a random mutation, resulting in a new allele being formed
- The antibiotic diffuses through the agar, so needs time to do so. As it diffuses through, it kills any bacteria
- Bacteria is spread onto the agar via the inoculating loop, which is then spread. Soak a paper disc in different antibiotics and place onto the agar plate. Tape the lid on and lightly incubate at 25 degrees for 48 hours. All aseptic techniques should be used
- Aseptic techniques
Comments
No comments have yet been made