Root tip squash- core practical

  • Created by: Emling
  • Created on: 22-03-19 09:40
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  • Root tip squash- Core Practical
    • There are no variables for this core practical as you are observing mitosis. Nothing will affect this (in terms of what causes it or stops it) it is just observation
    • Apparatus
      • Garlic roots
      • Hydrochloric acid
      • Toluidine blue
      • Distilled water
      • Test tubes
      • Pipet
      • Tweezers
      • Scaple
      • White tile
      • Microscope
    • Method
      • 1. Cut the very ends of the garlic root off with a scaple
      • 2. Place the root tips into 2cm cubed of hydrochloric acid in a test tube
      • 3. Place test tubes in a 60 degree water bath for 5 minutes so cell walls soften
      • 4. Take roots out of acid and rinse in cold water
      • 5. Place tips on microscope slide
      • 6. Gently break up the root tip to spread the cells out on slide
      • 7. Add a drop of toluidine blue to the cells and mop up excess with paper towel
      • 8. Place on cover slip and view under microscope

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