DNA probes

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  • Created by: emma
  • Created on: 16-04-13 19:28
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  • DNA probes
    • short single stranded section of DNA
      • labeled
        • radioactively: probe is made up of nucleotides which contain 32P. this will expose a photographic plate
          • site where probe binds can be identified
            • fluorescently: probes emit light under certain conditions
        • fluorescently: probes emit light under certain conditions
    • how do the probes work?
      • the probe has a base sequence complementary to the portion of the DNA sequence that makes up the gene which we want to find.
        • DNA to be tested split into its two seperate strands.
          • strands and probes mix. probe binds to complementary bases on one strand (DNA hydridisation)
            • site where probe binds can be identified

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