DNA profiling 0.0 / 5 ? BiologyForensicsA2/A-levelEdexcel Created by: francesca_321Created on: 07-12-15 11:41 What does PCR stand for? Polymerase Chain reaction 1 of 15 What is placed in the test tube for PCR? free mononucleotides, DNA primers and DNA polymerase 2 of 15 What is the first temperature the solution is heated to? 95 degrees celsius 3 of 15 Why? The DNA becomes denatured, H bonds break 4 of 15 What is the next temperature? 55 degrees celsius 5 of 15 What happens here? small pieces of DNA with fluorescent markers, attach at the start of the STR repeated sequence 6 of 15 What is the next temperature? heated to 70 degrees celsius 7 of 15 What attaches here? DNA polymerases and nucleotides are added extending the DNA from the Primer 8 of 15 What is the first stage of DNA profiling? Copy a sample of DNA using PCR 9 of 15 What happens to the DNA? IT is cut using restriction enzymes- endonucleases 10 of 15 What happens to the sample of DNA? Its run through an electrophoresis gel 11 of 15 What is the DNA attracted to? the anode because it is negatively charged. 12 of 15 What is the DNA transferred to? nylon or nictroclellulose membrane 13 of 15 Where is the membrane placed? in a bag with DNA probe 14 of 15 Why is a DNA probe important? It is radioactive. 15 of 15
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