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LOCATING AND SEQUENCING GENES

Recombinant DNA technology has enabled us to diagnose and treat many genetic disorders.
In order for us to do this it is necessary to know exactly where a particular gene is located.
To achieve this we use labelled DNA probes and DNA hybridisation.

A DNA probe…

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GEL ELECTROPHORESIS

Gel electrophoresis allows for the separation of DNA molecules based on their size

PROCESS

DNA samples are placed into wells at one end of the gel and covered in a buffer
solution.

An electric current is passed through the gel.

Each nucleotide in a molecule of DNA contains…

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DNA SEQUENCING

DNA sequencing is the process of determining the exact order of the bases A, T, C and G
in a piece of DNA.

PROCESS

The DNA to be sequenced is provided in singlestranded form. This acts as a
template upon which a new DNA strand is synthesised.

DNA…

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