Gel electrophoresis For SNAB/Edexcel. Topic 6 Gel electophoresis. 2.0 / 5 based on 1 rating ? BiologyA2/A-levelAll boards Created by: vithusaaCreated on: 18-12-11 15:30 DNA is cut into fragments using PCR (polymerase chain reaction) or restriction enzymes Fragments of double stranded DNA are loaded into wells of an agarose tank using a micropipette DNA and DNA fragments carry a negative charge. AN electric curretn is passed thorugh, so they move towards the positive electrode. Lighter fragments move faster and futher up the gel, whereas heavier once trave more slowly The fragments seperate into invisible bands DNA is transferred to a nylon/nitocellulose membrane placed over the gel as the fragments are invisible. The solution is drawn up through the solution DNA double strands split and stick oth the membrane The membrane is placed in a bag with DNA probe SIngle- stranded DNA probe binds to fragments with complementary sequence If the DNA probe is radioactive, X-ray film is used to detect fragments. If fluroscent, it is viewed under UV light 1 of 1
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