Microscopy - (Module 2.1.1)
Mindmap for microscopy.
Try to use and then cover it up and see what you can remember on a post-it note!
Happy revising beautiful, you'll do amazing! :)
- Created by: kizsidhu
- Created on: 18-03-18 16:43
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- Microscopy (Module 2.1.1)
- Light Compound Microscope
- inexpensive to buy & operate
- Small and portable
- Simple sample preparation
- Squash slides
- wet mount, squashed, cover slip
- Smear slides
- thin layer, cover slip
- wet mounts
- suspended in liquid, cover slip applied at angle to avoid bubbles (artefacts)
- dry mount
- sectioned, cover slip
- FSSM: flowers smell so much. fixing, sectioning, staining, mounting
- sectioned, cover slip
- Squash slides
- vaccum not needed
- 2000x magnification
- 200nm resolution
- specimen can be living or dead
- natural colour of sample is seen or a stain is used
- Gram Staining
- gram negative or gram positive bacteria
- Acid fast staining
- mycobacteria from normal bacteria
- Other stains
- positive
- crystal violet
- methylene blue
- negative
- congo red
- nigrosin
- positive
- Gram Staining
- Electron Microscopy
- SEM (scanning electron microscope)
- expensive to buy & operate
- Needs to be installed
- complex sample preparation
- sample has to be dead
- often distorts sample
- 500,000 magnification
- 3-10nm resolution
- Black and white image produced
- 3D dimensional image
- expensive to buy & operate
- TEM (transmission electron microscope)
- expensive to buy & operate
- Needs to be installed
- 500,000 magnification
- complex sample preparation
- sample has to be dead
- often distorts sample
- black and white image produced
- 2D Image produced
- 0.5nm resolution
- expensive to buy & operate
- SEM (scanning electron microscope)
- Laser scanning confocal microscope
- specimen treated with a fluorescent dye
- light of a longer wavelength and lower energy is emitted to produce a magnified image
- single spot of focused light
- filtered through a pinhole aperture
- only light radiated very close to focal plane detected
- laser used instead of light to get higher intensity
- thin specimen = high resolution
- non invasive
- used to diagnose eye problems
- used in drug development
- Light Compound Microscope
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