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Monday, 10 September 2012

F211 Module 1 Cells and the Microscope

Weekly Learning Outcomes

Students should be able to:
State the resolution and magnification that can be achieved by a light microscope, a transmission
electron microscope (TEM) and a scanning electron microscope (SEM).
Explain the difference between magnification and resolution.…

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1. Staining ­ Coloured stains are chemicals that bind to chemicals on or in the specimen. This allows
the specimen to be seen. Some stains bind to specific cell structures. One example is iodine, which
stains starch a dark blue/ black colour.
2. Sectioning ­ Specimens are embedded in wax.…

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Electrons are not visible to the human eye. The image produced from the electron beam is
projected onto a screen or photographic paper to make a black-and-white image.
Such images are called electron micrographs.
The resolution of an electron microscope is about 500000 times greater than that of the human…

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Magnification ­ The number of times greater an image is than the object.

Resolution ­ The ability to distinguish two separate points as distinct from each other.

Cell surface membrane ­ The membrane that surrounds every cell, forming the selectively permeable
boundary between the cell and the environment.



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