UCL Transamination Practical

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1. The dinitrophenylhydrazine...

  • runs irregularly near the top so ignore the top spots and concentrate on the pyruvate DNP and 2-oxoglutarate DNP spots
  • runs irregularly near the top so read it as being the same level as either the pyruvate DNP and 2-oxoglutarate DNP spots
  • often produces two spots close together (isomers)
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2. What can we derive is in position 8?

  • oxoglutarate
  • pyruvate

3. what’s in position 4?

  • glutamate (technically shouldn’t be there, it’s because of limiting factors)
  • Pyruvate (technically shouldn't be there, because of limiting factors)

4. After the extracts have been added (which are added after the reactants), ethyl acetate is added to tubes 5- 10 and the tubes are shaken well. What does this do?

  • extracts the 2,4 dinitrophenyl-hydrazones
  • separates the ethyl acetate

5. What is solvent system II?

  • n-butanol: ethanol: aqueous ammonia (conc) 70: 10: 20 (oxoacids)
  • ethanol: aqueous ammonia (conc) 70:30 (oxoacids)

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