1. The dinitrophenylhydrazine...
- runs irregularly near the top so ignore the top spots and concentrate on the pyruvate DNP and 2-oxoglutarate DNP spots
- runs irregularly near the top so read it as being the same level as either the pyruvate DNP and 2-oxoglutarate DNP spots
- often produces two spots close together (isomers)
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Other questions in this quiz
2. What can we derive is in position 8?
3. what’s in position 4?
- glutamate (technically shouldn’t be there, it’s because of limiting factors)
- Pyruvate (technically shouldn't be there, because of limiting factors)
4. After the extracts have been added (which are added after the reactants), ethyl acetate is added to tubes 5- 10 and the tubes are shaken well. What does this do?
- extracts the 2,4 dinitrophenyl-hydrazones
- separates the ethyl acetate
5. What is solvent system II?
- n-butanol: ethanol: aqueous ammonia (conc) 70: 10: 20 (oxoacids)
- ethanol: aqueous ammonia (conc) 70:30 (oxoacids)