UCL Transamination Practical

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  • Created by: cgeorge
  • Created on: 14-04-14 20:07

1. What is solvent system II?

  • n-butanol: ethanol: aqueous ammonia (conc) 70: 10: 20 (oxoacids)
  • ethanol: aqueous ammonia (conc) 70:30 (oxoacids)
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Other questions in this quiz

2. Tubes 3 and 7, the reactants are pyruvate and glutamate and the products are oxoglutarate and alanine. What was the reaction?

  • no reaction
  • transamination
  • deamination

3. Which of these is true?

  • in this experiment, we separate amino acids on one plate using one solvent mixture and oxoacid derivatives on another plate with a different solvent mixture
  • in this experiment, we separate amino acids and oxoacids on the same plate, due to the ability of one solvent mixture to separate these simultaneously.

4. What does the second option of these two routes do?

  • it is oxidatively deaminated, which releases an ammonium ion
  • it can contribute its amino group directly for synthesis of urea
  • it contributes its ammonium ion directly for synthesis of urea

5. What do transaminases have as a cofactor?

  • tightly bound phosphate which is derived from Vitamin A6
  • tightly bound pyridoxal phosphate which is derived from vitamin B6
  • loosely bound phosphate which is derived from Vitamin B6

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