7. Tubes 3 and 7, the reactants are pyruvate and glutamate and the products are oxoglutarate and alanine. What was the reaction?
transamination
deamination
no reaction
8. What does this produce?
A new amino acid and a new 2-oxo acid
Two amino acids and a floating amino group
9. After the extracts have been added (which are added after the reactants), ethyl acetate is added to tubes 5- 10 and the tubes are shaken well. What does this do?
extracts the 2,4 dinitrophenyl-hydrazones
separates the ethyl acetate
10. What’s in position 2?
Glutamate
Pyruvate
11. Pyruvate DNP…
often produces two spots close together (isomers). More rarely, 2 spots are seen with 2-oxoglutarate DNP as well
runs irregularly near the top so ignore the top spots and concentrate on the pyruvate DNP and 2-oxoglutarate DNP spots
12. How are the amino acids visualised?
they are yellow and can be seen when sprayed with ninhyrdin (and heated)
they are purple and can be seen when sprayed with ninhydrin (and heated)
they are purple and can be seen directly
13. What does the first option of these two routes do?
it is transaminated and directly contributes its amino group to synthesis of urea
it is oxidatively deaminated, which releases an ammonium ion
it is oxidatively deaminated to directly contribute its amino group to synthesis of urea
14. What does glutamate dehydrogenase do?
catalyses the oxidative deamination of glutamate
catalyses the oxidation of glutarate
moves amino acids between glutamate and glutamic acid
15. What’s in position 6?
DNPH (buffer compounds)
Alanine
16. Tube 10 gives a standard of what?
the DNPH alone
the 2,4 dinitrophenyl-hydrazone of pyruvate
the 2,4, DNPH of 2 oxo glutarate
17. What’s in position 9?
Oxoglutarate
pyruvate
18. What do transaminases do?
move amino acids between amino groups and oxoacids
move amino groups between amino acids and oxoacids
move phosphates between amino acids and oxoacids
19. Glutamate dehydrogenase is unusual in that it..
uses either NAD+ or NADP+ as a coenzyme
uses both FAD+ and NAD+ as a coenzyme
20. Tube 10, the reactants were none and none. What was the reaction?
