UCL Transamination Practical

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1. After the extracts have been added (which are added after the reactants), ethyl acetate is added to tubes 5- 10 and the tubes are shaken well. What does this do?

  • extracts the 2,4 dinitrophenyl-hydrazones
  • separates the ethyl acetate
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2. Tubes 4 and 8, the reactants are NAD+ and glutamate and the products are oxoglutarate. What was the reaction?

  • deamination
  • transamination
  • no reaction

3. What does the first option of these two routes do?

  • it is oxidatively deaminated, which releases an ammonium ion
  • it is oxidatively deaminated to directly contribute its amino group to synthesis of urea
  • it is transaminated and directly contributes its amino group to synthesis of urea

4. (transaminases) Which amino acid has an amino group removed and where is it transferred to?

  • the alpha amino acid has an amino group removed and transferred to a beta amino acid
  • the alpha amino acid has an amino group removed and transferred to a 2-oxoacid
  • the beta amino acid has an amino group removed and transferred to a 2-oxoacid

5. What happens to the toxic ammonium ion released from the oxidative deamination of glutamate?

  • it is transported (as glutamine) to the liver, where it is converted to carbamoyl phosphate, which enters the urea cycle
  • it is transported (as glutamate) to the liver, where it is converted to carboxyl phosphate, which enters the urea cycle

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