UCL Transamination Practical

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1. After the extracts have been added (which are added after the reactants), ethyl acetate is added to tubes 5- 10 and the tubes are shaken well. What does this do?

  • extracts the 2,4 dinitrophenyl-hydrazones
  • separates the ethyl acetate
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2. (Use the drawings of your plates for the rest of the questions.) What's in

  • Nothing (no amino acids produced)
  • Pyruvate
  • Glutamate

3. What’s in position 6?

  • DNPH (buffer compounds)
  • Alanine

4. How are the amino acids visualised?

  • they are purple and can be seen when sprayed with ninhydrin (and heated)
  • they are purple and can be seen directly
  • they are yellow and can be seen when sprayed with ninhyrdin (and heated)

5. What are the two routes for this aim of the interconverting?

  • one is glutamate and one is aspartate
  • one is pyruvate and one is aspartate
  • one is glutamate and one is pyruvate

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