UCL Transamination Practical

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1. What is the purpose of TLC (thin layer chromatography)?

  • it is a quick and convenient way of separating many types of compound
  • it is a quick and convenient way of identifying the presence of substance
  • it is a length but very precise way to separate many types of compound
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2. Tube 9, the reactants were oxoglutarate and the products are oxoglutarate. What was the reaction?

  • deamination
  • no reaction
  • transamination

3. Tubes 3 and 7, the reactants are pyruvate and glutamate and the products are oxoglutarate and alanine. What was the reaction?

  • transamination
  • deamination
  • no reaction

4. What does the second option of these two routes do?

  • it is oxidatively deaminated, which releases an ammonium ion
  • it can contribute its amino group directly for synthesis of urea
  • it contributes its ammonium ion directly for synthesis of urea

5. What’s in position 9?

  • Oxoglutarate
  • pyruvate

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