UCL Transamination Practical

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1. Tubes 1 and 5, the reactants are pyruvate and the products are pyruvate. What was the reaction?

  • no reaction
  • transamination
  • deamination
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2. What can we derive is in position 3?

  • alanine (and some left over glutamate)
  • pyruvate (and some left over glutamate)

3. What’s in position 6?

  • DNPH (buffer compounds)
  • Alanine

4. After the ethyl acetate is added to tubes 5-10, all ten tubes were centrifuged. What was the purpose of this?

  • to remove precipitated protein and to separate the ethyl acetate (5-10)
  • to extract the ethyl acetate and separate the 2,4 dinitrophenyl-hydrazones
  • to remove ethyl acetate (5-10) and separate the 2,4 dinitrophenyl-hydrazones

5. Which of these is true?

  • in this experiment, we separate amino acids on one plate using one solvent mixture and oxoacid derivatives on another plate with a different solvent mixture
  • in this experiment, we separate amino acids and oxoacids on the same plate, due to the ability of one solvent mixture to separate these simultaneously.

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