UCL Transamination Practical

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1. How are the amino acids visualised?

  • they are purple and can be seen when sprayed with ninhydrin (and heated)
  • they are purple and can be seen directly
  • they are yellow and can be seen when sprayed with ninhyrdin (and heated)
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Other questions in this quiz

2. What is the purpose of TLC (thin layer chromatography)?

  • it is a length but very precise way to separate many types of compound
  • it is a quick and convenient way of separating many types of compound
  • it is a quick and convenient way of identifying the presence of substance

3. Tubes 4 and 8, the reactants are NAD+ and glutamate and the products are oxoglutarate. What was the reaction?

  • deamination
  • transamination
  • no reaction

4. Tube 5 gives you a standard of what?

  • the 2,4 dinitrophenyl-hydrazone of pyruvate
  • the 2 oxoglutarate
  • the DNPH alone

5. Glutamate dehydrogenase is unusual in that it..

  • uses either NAD+ or NADP+ as a coenzyme
  • uses both FAD+ and NAD+ as a coenzyme

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