Topic 6: PCR

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Created by: sakiya
Created on: 23-01-19 13:02

What happens during PCR?

Many copies of a DNA fragment are formed.

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What is needed for PCR?

Template DNA, DNA polymerase, primers and a buffer solution

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What is the template DNA used for?

This is the DNA that will be copied.

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What is DNA polymerase needed for?

This is the enzyme which binds free nucleotides to the section of DNA that is copied.

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What is the process annealing?

This is where the primers bind to the complementary sections of DNA by forming hydrogen bonds this occurs at 55 degrees (they bind to the end of the DNA fragments).

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What is the buffer solution needed for?

This maintains the pH at levels at which DNA polymerase can function effectively.

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What happens at the first stage of PCR?

The DNA is heated up to 95 degrees to break up the hydrogen bonds and separate the DNA into two strands

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What happens directly after annealing?

The temperature is then increase to 72 degrees at which DNA polymerase can now work at its optimum and attach free nucleotides to the single stranded fragment. This forms two new strands of DNA (one new and one old strand).

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Other cards in this set

Card 2

Front

What is needed for PCR?

Back

Template DNA, DNA polymerase, primers and a buffer solution

Card 3

Front

What is the template DNA used for?

Back

Card 4

Front

What is DNA polymerase needed for?

Back

Card 5

Front

What is the process annealing?

Back

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Topic 6: PCR