What does frequency within the visible light and UV rays range show
change in electron distribution
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What does frequency within the IR range show?
arrangement of bonds
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What does frequency within the NMR range show?
nuclear spin.
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What is constructive interference?
When two waves with the same phrase and freq form a wave with the same freq but higher amplitude.
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What is destructive interference?
When two waves have different phrase but same freq and effectively cancel each other out.
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What is spectroscopy?
Matching freq of electromagnetic to identify sample.
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What are chromophores?
Natural substances that are coloured due to how they transmit and absorb certain freq of light.
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Why do we typically use UV-vis for detecting proteins?
As three aa have double bonds, tryptophan, tyrosine and phenylalanine that absorb at certain light so if you know how many of that aa you can know how much protein there is.
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What is the beer-lambert law and what is it used for?
absorbance=E x l x c . L-->length light passes through solution cm. C--> conc in molar. The law shows the absorbance of light and the conc of the solution absorbing it.
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Describe IR spec.
uses longer wavelengths and lower freq than uv-vis. it is associated with bond vibration, stretching and bending. Limited to macromolecules due to low resolution.
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Describe fluorescence spec.
excite samples it absorbes light at certain wavelength and gives light out at anout (detector at 90degrees). Very sensitive can detect a single molecule. fluorophores typically contain multiple aromatic groups.
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Describe NMR spec
very insensitive. The nuclear spin of an atom shows the chemical environments as it effects freq of nuclei. atomic resolution. individual peaks are one atom. so high resolution power
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what is mass spec
measure mass of a molecule to identify a sample
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what is x-ray crystallography
pattern of crystalline solid exposed to x-ray radiation to identify it's 3D structure.
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Other cards in this set
Card 2
Front
What is the wavelength of visible light?
Back
0.5x10^-6m
Card 3
Front
What does frequency within the visible light and UV rays range show
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