what are the disk-shaped structures in chloroplasts?
Thylakoids
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what are these called when arranged in stacks?
Grana
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what is an advangtage of having lots of grana?
provide large surface area for chlorophyll molecules
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why does a chlorophyll solution appear red, not green when light shines through it
the chlorophyll molecules have been photoionised (= absorbs light & loses TWO electrons, becoming positively charged)
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what happens to the electrons after photoionisation happens in a chloroplast?
the pass along electron carriers (electron transfer chain) losing energy as they go
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What is the next step in the light-Dependant reaction, after photoionisation?
photolysis = water broken down --> 2Protons + 2electrons + 1/2 Oxygen
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what is role of the 2 electrons from photolysis ?
To replace the 2 lost from photoionisation
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what happens when the second chlophyll molecule is photoionised?
the 2 electrons lost are used with the 2 protons from photolysis of water to produced REDUCED NADP (NADP + 2H+ +2e- --> reduced NADP)
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What happens to energy lost from electrons as they pass through electron transfer chain?
the energy is used to actively transport protons from stroma across thylakoid membrane into spaces between thylakoids - creating higher conc of protons inside thylakoid than stroma
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what happens to protons when proton conc increases within thylakoid?
they diffuse back out down conc gradient to stroma - they diffuse through molecules of ATP SYNTHASE casuing protein to change shape and SPIN.
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What is the importance of ATP Synthase Spinning?
it provides energy for synthesis of ATP from ADP + Pi
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how can we observe the light-dependant reaction in the lab?
isolated chloroplasts in buffer absorb water via osmosis & burst, releasing thylakoids into solution. Blue dye (DCPIP) is added & e- are transferred to the dye rather than NADP causing DCPIP to be reduced and go from blue to colourless
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Other cards in this set
Card 2
Front
what are these called when arranged in stacks?
Back
Grana
Card 3
Front
what is an advangtage of having lots of grana?
Back
Card 4
Front
why does a chlorophyll solution appear red, not green when light shines through it
Back
Card 5
Front
what happens to the electrons after photoionisation happens in a chloroplast?
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