Genetics In Vitro mutagenesis L8

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  • Created by: Alex
  • Created on: 12-04-13 01:55
Mutagenesis
process by which the genetic information of an organism is changed in a stable manner, resulting in a mutation
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ADME characteristics
Absorption, Distribution, Metabolism and Excretion - describes the disposition of a pharmaceutical compound within an organism.
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Oligonucleotide
Short polymer of nucleic acids of 50bp or less - often are primers in PCR reactions
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Reverse genetics
Starting with a gene of interest which is cloned and worked toward a mutant phenotype
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In vitro mutagenesis
the production of random or specific mutations in a segment of cloned DNA
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Uses of invitro mutagenesis
Study gene function - determind the role of several a.acids or a protien by enhancing or modifying its function
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Random in vitro plasmid mutations
Identifies location of boundaries of a particular fuction of a DNA segment. identified by genetic screen. First step when little is known about the function encoded by the DNA of interest. Narrows focus from a large gene to a smaller region
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Site-Directed in vitro plasmid mutations
specific location of mutation. - defines role of specific sequences - powerful tool in protein function analysis - allows changes in protein structure.
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How to mutate an in vitro plasmid
Restriction endonucleases. linker insertion mutagenesis. nested deletions - unidirectional ad bideirectional. Oligonucleotide directed mutagenesis. cassette replacement. PCR directed mutagenesis
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Over view of plasmid mutation steps
1. mutate plasmid DNA in vitro. 2.transfrom bacterial cells with the mutated plasmid DNA. 3,Screen for colonies that contain mutant plasmid DNA
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Single-nucleotide polymorphism
DNA sequence variation occurring when a single nucleotide adenine (A), thymine (T), cytosine (C), or guanine (G]) in the genome (or other shared sequence) differs between members of a species or paired chromosomes in an individual
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Insertational mutagenesis
Mutation caused by the insertion of new genetic material into a normal gene. can occur naturally, mediated by virus or transposon, or can be artificially created for research purposes in the lab.
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Other cards in this set

Card 2

Front

ADME characteristics

Back

Absorption, Distribution, Metabolism and Excretion - describes the disposition of a pharmaceutical compound within an organism.

Card 3

Front

Oligonucleotide

Back

Preview of the front of card 3

Card 4

Front

Reverse genetics

Back

Preview of the front of card 4

Card 5

Front

In vitro mutagenesis

Back

Preview of the front of card 5
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