ELISA

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1. In a sandwich ELSIA, what would happen there was no washing steps after the detection antibody was added to the well?

  • All wells, including the positive control wells, would not show a colour change when substrate was added
  • All wells, including the negative control wells, would show uniform overdevelopment
  • The ELISA would develop normally (positive control is positive, negative control is negative)
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Other questions in this quiz

2. In a sandwich ELSIA, what would happen if the blocking step was omitted?

  • All wells, including the negative control wells, would show uniform overdevelopment
  • The ELISA would develop normally (positive control is positive, negative control is negative)
  • All wells, including the positive control wells, would not show a colour change when substrate was added

3. The direct ELISA is used to test for the presence of specific antibodies in a patient’s serum.

  • False
  • True

4. The direct sandwich ELISA requires as a first step to coat the well with

  • capture antibody
  • patient serum
  • antigen
  • complement

5. In the indirect ELISA, the enzyme linked antibody will attach to

  • the blocking reagent such as BSA
  • the constant region of the patient antibody
  • the patient antigen
  • the variable region of the patient antibody

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