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1. In a sandwich ELSIA, what would happen there was no washing steps after the detection antibody was added to the well?
- All wells, including the positive control wells, would not show a colour change when substrate was added
- All wells, including the negative control wells, would show uniform overdevelopment
- The ELISA would develop normally (positive control is positive, negative control is negative)
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2. In a sandwich ELSIA, what would happen if the blocking step was omitted?
- All wells, including the negative control wells, would show uniform overdevelopment
- The ELISA would develop normally (positive control is positive, negative control is negative)
- All wells, including the positive control wells, would not show a colour change when substrate was added
3. The direct ELISA is used to test for the presence of specific antibodies in a patient’s serum.
4. The direct sandwich ELISA requires as a first step to coat the well with
- capture antibody
- patient serum
- antigen
- complement
5. In the indirect ELISA, the enzyme linked antibody will attach to
- the blocking reagent such as BSA
- the constant region of the patient antibody
- the patient antigen
- the variable region of the patient antibody
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