DNA technology

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  • Created by: Mark18
  • Created on: 12-05-14 17:00
What is the role of Reverse Transcriptase
Forms single stranded complementary DNA from mRNA
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What does DNA polymerase do
It causes nucleotides to join together as a strand
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What do restriction endonucleases do
Cut double stranded DNA at a specific recognition sequences
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State the 3 stages of the polymerase chain reaction including temperature and what occurs at each stage
95 degrees- so that the the two strands in DNA seperate, 55 degrees- to allow primers to join to the complementary bases, 72 degrees so that DNA polymerase can join the strands together
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What does DNA ligase do
Joins up the phosphate-sugar framework of the two sections of DNA
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What is the purpose of a gene marker
Allows us to identify host cells which have successfully taken up the correct gene
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What is a vector
Something that carries DNA into cells for replication e.g. a plasmid
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What is transformation
Delivering a gene into a living cell
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Other cards in this set

Card 2

Front

What does DNA polymerase do

Back

It causes nucleotides to join together as a strand

Card 3

Front

What do restriction endonucleases do

Back

Preview of the front of card 3

Card 4

Front

State the 3 stages of the polymerase chain reaction including temperature and what occurs at each stage

Back

Preview of the front of card 4

Card 5

Front

What does DNA ligase do

Back

Preview of the front of card 5
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