DNA technology

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Created by: Mark18
Created on: 12-05-14 17:00

What is the role of Reverse Transcriptase

Forms single stranded complementary DNA from mRNA

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What does DNA polymerase do

It causes nucleotides to join together as a strand

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What do restriction endonucleases do

Cut double stranded DNA at a specific recognition sequences

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State the 3 stages of the polymerase chain reaction including temperature and what occurs at each stage

95 degrees- so that the the two strands in DNA seperate, 55 degrees- to allow primers to join to the complementary bases, 72 degrees so that DNA polymerase can join the strands together

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What does DNA ligase do

Joins up the phosphate-sugar framework of the two sections of DNA

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What is the purpose of a gene marker

Allows us to identify host cells which have successfully taken up the correct gene

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What is a vector

Something that carries DNA into cells for replication e.g. a plasmid

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What is transformation

Delivering a gene into a living cell

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Card 2

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What does DNA polymerase do

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It causes nucleotides to join together as a strand

Card 3

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What do restriction endonucleases do

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Card 4

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State the 3 stages of the polymerase chain reaction including temperature and what occurs at each stage

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Card 5

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What does DNA ligase do

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DNA technology