B1 Light Microscopy OCR Gateway (9-1)

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  • Created by: Aleena
  • Created on: 03-04-18 11:31
What is the eyepiece lens?
looked through to see the image & also magnifies the image
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What is the objective lens?
magnifies the image, usually there are 3 different objective lenses e.g. x4, x10, x40
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What is the Stage?
Platform that supports the slide
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What is the clip?
Holds the slide in place
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What is the handle?
So you can carry the microscope with
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What is the lamp?
It shines light through the slide so the image can be seen more easily
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What are the focusing knobs?
Moves the stage up & down to bring to image into focus
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How do you prepare specimens to view under the light microscopes?
1)Take a thin slice of it 2)Use pipette to drop water/mountant on a slide 3) use tweezers to put specimen on slide,add drop of stain if needed 4)Place on top the cover slip using mounted needle
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What is a mountant?
a clear,gloopy liquid
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why do you put water or mountant on the slide?
to secure the specimen in place
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when do you use a stain?
When specimen is completely transparent or colourless-so you ca see it easier
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Give some examples of stains
Eosin-stains cytoplasm Methylene Blue stains DNA
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What is a cover slip?
a sqaure of thin, transparent plastic/glass
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why do carefully lower the cover slip over the specimen?
to ensure no air bubbles get trapped
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How do you correctly use a light microscope to view the prepared specimen?
watch the revision video
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Why is a higher magnification not always a good thing?
If your specimen is relatively big you might not be able to see the whole thing, It can also be difficult to focus at high magnifications
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What do you after you are happy with what you can see in microscope?
you produce a scientific drawing of your specimen
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Other cards in this set

Card 2

Front

What is the objective lens?

Back

magnifies the image, usually there are 3 different objective lenses e.g. x4, x10, x40

Card 3

Front

What is the Stage?

Back

Preview of the front of card 3

Card 4

Front

What is the clip?

Back

Preview of the front of card 4

Card 5

Front

What is the handle?

Back

Preview of the front of card 5
View more cards

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