Slides and photomicrogrpahs - Staining
- many structures are colourless and transparent
- if transparent they will appear white
- coloured chemicals will give artifical colours to the specimen (staining)
- codes used; st,wm,cs,ls,sq,sm
How to prepare a specimen:
- fixing - cells and other elements in the specimen are preserved in a "life-like" state
- processing - water is removed from the specimen. Duration will depend on nature and size
- embedding - placed in wax-filled moulds. Cassette is placed on top of mould, with more wax.
- sectioning - cut on a "microtome". Thickness of 3-5 micrometres, single layer of cells
- staining - H&E is most common. Cell nuclei are stained blue and cytoplasm is pink with H&E.
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