Glycogen Depletion - enough to last 90 minutes, when depleted body tries to metabolise fats, unable to use it on its own.
Lactic Acid Bulid Up - accumulation of Lactic Acid releases Hydrogen ions, increase in acidity of blood, inhibits enzyme action and breakdown of glucose, irritates nerve endings, causes pain.
Reduced Rate of ATP Synthesis - when ATP and PC are depleted, difficult to sustain muscular contractions.
Dehydration - Blood viscosity increases, effect on blood flow to working muscles, blood pressure reduces. Reduction in sweating to prevent water loss, increases core body temperature
Reduced levels of Calcium - needed for muscular contractions, increase in hydrogen ions decreases amount of calcium released from sarcoplasmic reticulum.
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