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Stages of Cell Division
Cell division occurs in two main stages
Nuclear Division (karyokinesis): nucleus divides either in
mitosis or meiosis
Cell Division (cytokinesis): follows nuclear division, it is
where the whole cell divides
Before a nucleus divides its DNA must be replicated.
This makes sure that daughter cells have genetic
information to produce enzymes and other proteins.
DNA replication is the process of making copies
(duplication) of DNA molecules in the cell nucleus.…read more

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Semiconservative Replication
Semiconservative replication of DNA produces two
copies of DNA that each contain one of the original
strands and one entirely new strand.
Describes the mechanism by which DNA is replicated in
all known cells.…read more

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Requirements for Replication
Four types of nucleotide must be present with their
bases adenine, guanine, cytosine and thymine
Both DNA molecule strands must act as a template for
the attachment of nucleotides
The enzyme DNA polymerase is needed to catalyze the
A source of energy is required eg. ATP…read more

Slide 5

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Semiconservative Replication Process
The enzyme DNA helicase breaks hydrogen bonds
linking base pairs of DNA
The double helix separates into two strands & separates
Each exposed polynucleotide strand acts as a template
to which complementary nucleotides are attracted
Energy is used to activate the nucleotides
Activated nucleotides are joined together by the
enzyme DNA polymerase to form missing
polynucleotide strand
Each new DNA molecule has one original DNA strand
and one new DNA strand…read more

Slide 6

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1. DNA helicase breaks H-bonds
linking base pairs
2. Therefore double helix
separates and unwinds
3. Exposed polynucleotide acts as
a template to which
complementary nucleotides are
4. Energy used to activate
5. Activated nucleotides joined by
DNA polymerase
6. Each of 2 new DNA molecules
contains half original DNA…read more

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