AQA AS Biology Unit 1: Indentification of Biologically Important Compounds

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  • Created on: 22-08-13 16:11
Preview of AQA AS Biology Unit 1: Indentification of Biologically Important Compounds

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Identification of Biologically Important Compounds
Starch
Add substance under test to a test tube
Add 2 drops of iodine in potassium iodide solution
Results: blue/black colour indicates present of starch
Reducing Sugar
Add substance under test to a test tube
Add a few drops of Benedict's Reagent
Shake and boil gently
Results: brick red precipitation indicates sugar
green precipitate indicates a small amount
yellow precipitate is in-between
Non-reducing Sugars
Add substance under test to a test tube
Add a few drops of hydrochloric acid
Boil gently and cool under running water
Neutralise with sodium hydrogen carbonate until the
effervescence stops
Add a few drops of Benedict's Reagent
Results: brick red precipitation indicates sugar
green precipitate indicates a small amount
yellow precipitate is in-between

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Identification of Biologically Important Compounds
Protein
Add substance under test to a test tube
Add a few drops of Biuret reagent
Warm gently
Results: Purple colour indicates protein
If a yellow precipitate is obtained there is a lot of sugar
present masking the result.
Dip an albustix reagent strip into a fresh suspension with
reagent in it.…read more

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