Biology - electron microscope (TEM and SEM)

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Biology - electron microscope (TEM and SEM)

Advantages

  • TEM generate high resolution cross-sections of objects
  • SEM display enhanced depth to map the surface of objects in 3D
  • Higher resolution for more detailed and clearer images
  • Higher magnification to detect more structures

Disadvantages

  • Both electron microscopes must be a vacuum for it to work. Therefore, living specimens cannot be observed
  • Complex staining process
  • TEM specimens must be thin for the electrons to penetrate
  • May contain artefacts, things resulting from the way it was prepared. May appear on finished photomicrograph but not part of specimen - makes it hard to be sure that what we see really exists
  • TEM resolving power cannot always be achieved because of preparation and risk of destroying specimen

Evaluation

The electron microscope is used by experienced, highly trained scientists working in a lab. They provide detailed images of specimens. However, they are not suitable for beginners and students and have limitations 

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