Biology - Compare the SEM and TEM

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Type of electrons

  • SEM: Scattered, scanning electrons
  • TEM: Transmitted electrons

High tension

  • SEM: ~ 1-30kV
  • TEM: ~60-300kV

Specimen thickness

  • SEM: Any
  • TEM: Typically less than 150nm

Type of information

  • SEM: 3D image of surface
  • TEM: 2D projection image of inner structure

Maximum magnification

  • SEM…

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