RNA and protein synthesis

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  • RNA and protein synthesis
    • protein synthesis
      • stage 1: transcription
        • 1. RNA polymerase attaches to DNA double helix and breaks H bonds (unzips)
          • 2. mRNA makes a copy
            • 3. RNA polymerase lines up free RNA nucleotides alongside exposed bases on the template strand
              • complementary base pairing
              • 4. joined up by RNA polymerase forming an mRNA molecule
                • 5. H bonds reform and strands coil into a double helix
                  • 6. stop signal stops more RNA being made
                    • 7. mRNA moves out of nuclear pores and attaches to the ribosome in the cytoplasm
        • in eukaryotes transcription results in the production of pre-mRNA.
          • splicing removes introns and joins exons
        • stage 2: translation
          • 1. mRNA attaches to ribosome and tRNA carries amino acids to it
            • 2. tRNA with an anticodon that's complementary to the first codon on the mRNA attaches itself by complementary base pairing
              • 3. a second tRNA molecule attaches itself to the next codon
                • 4. two amino acids attached to the tRNA are joined by peptide bonds
    • RNA molecules
      • messenger RNA
        • made during transcription
        • carries genetic code from DNA to ribosomes where it's used to make a protein during translation
        • single polynucleotide strand
        • groups of three bases are called codons
      • transfer RNA
        • involved in translation. carries amino acids to ransoms
        • single polynucletidestrand that's folded into a clover
        • H bonds between specific bas pairs hold it in shape
        • every sequence of 3 bases at one end is called an anticodon and an amino acid binding site at the other end
    • genome
      • complete set of all genes in an organism
      • proteome
        • compleet set of all proteins an organism can produce

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