The Lac Operon
- Created by: Ellie0o0o
- Created on: 22-04-15 15:05
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- Lac Operon
- Cis-acting elements + protein-coding genes
- Lac ZYA genes
- A repressor
- beta-galactosidadse
- a permease
- A transacetylase
- Cis-acting: Lac I
- Repressor
- Lac ZYA genes
- A repressor
- beta-galactosidadse
- a permease
- A transacetylase
- Operator sites overlaps promoter site for Lac Operon
- Monomer with multiple domains
- N-terminal binding domain
- Contains 2 alpha helices which bind to the DNA major groove
- Hinge
- Core
- Forms a dimer. Hinge allows both monomers to bind at once
- N-terminal binding domain
- Lac ZYA genes
- Repressor
- Lac ZYA genes
- Lactose inducer
- Prevents repressor activity
- Disrupts hinge region of repressor
- LacZYA expressed at basal levels in presence of represser
- Very unstable mRNA so levels can rapidly alter
- There are actually 2 operator regions
- 2 dimers form a tetramer
- 1 dimer binds each operator region
- DNA forms a loop
- cAMP Repressor Protein (CRP) can bind to an adjacent sequence and activate transcription when activated by cAMP
- Cis-acting elements + protein-coding genes
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