BMS1031 Bacterial Genetics Example Q's

?
which codon serves as the start codon in mRNA for translation?
AUG
1 of 19
Mutation of an adenine bas to guanine base in DNA is called...
a transition mutation
2 of 19
Single base substitution in the codon UUA (leu) to form UCA (ser) represents a...
missense mutation
3 of 19
The ligand that binds to E.coli Lacl repressor is:
lactose (allolactose)
4 of 19
The ligand that binds to the E.coli CRP (CAP) protein is:
cyclic AMP
5 of 19
Mutations in the Lacl gene, encoding the Lacl repressor proteins:
generally lead to constitutive expression of the lac operon
6 of 19
Mutations in lacO, the operator of the lac operon prevent:
binding of Lacl repressor
7 of 19
cis-dominant mutations:
only affect expression of genes on the same DNA molecule
8 of 19
Repressor proteins:
A and C
9 of 19
How many different transcription factors are bound to the E.coli lac operon promoter region when both lactose and glucose are present in the growth medium?
none
10 of 19
When E.coli is grown on a mixture of lactose and glucose?
glucose is used first
11 of 19
cis-acting operator DNA sequences generally display:
dyad symmetry
12 of 19
The trp operon attenuation mechanism..
relies on the coupling of transcription and translation
13 of 19
Horizontal (lateral) gene transfer in bacteria can occur via:
cojugation
14 of 19
Lambda bacteriophage:
can enter either a lytic or lysogenic growth phase
15 of 19
Generalised recombination:
requires extensive sequence similarity between two DNA sequences
16 of 19
To express a eukaryotic gene in a bacterium:
A and B
17 of 19
cDNA cloning:
requires the enzyme reverse transcriptase
18 of 19
Which amino acid does the tRNA 5'-AAC-3' code?
Lysine (UUA, CUU etc)
19 of 19

Other cards in this set

Card 2

Front

Mutation of an adenine bas to guanine base in DNA is called...

Back

a transition mutation

Card 3

Front

Single base substitution in the codon UUA (leu) to form UCA (ser) represents a...

Back

Preview of the front of card 3

Card 4

Front

The ligand that binds to E.coli Lacl repressor is:

Back

Preview of the front of card 4

Card 5

Front

The ligand that binds to the E.coli CRP (CAP) protein is:

Back

Preview of the front of card 5
View more cards

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