Gene Technology-B6
- Created by: Shannon
- Created on: 10-06-13 09:30
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- Gene Technology
- Restriction enzymes cut open DNA-leave unpaired bases-act as "sticky ends"
- Ligase enzymes join DNA strands-sticky ends join by complimentar-y base pairing
- DNA fingerprinting-good for finding criminals, bad as they assess disease-could withhold life insurance
- Stages of production: extract DNA sample; fragmenting DNA using restriction enzymes; separate fragments using with electrophores-is; make fragments visible using a radioactive probe
- Assaying technique: genes added to make bacteria resistant to antibiotics- flooded with antibiotics-grown on nutrient agar containing antibiotic
- Bacteria that survive are chosen-used to determine whether a bacterium has been taken up by a plasmid
- DNA loops-plasmids-found in cytoplasm of bacteria, used as vectors for genes
- Bacteria and insulin-cultured by cloning
- Cut gene for insulin, open loop of DNA, insert insulin into loop, insert loop into bacteria
- Achieved using enzymes-cloning produces identical copies
- Genetic engineering-genetic code is universal: why this is possible
- Organism that receives gene-transgenic organism
- Main stages: identify and remove gene, cut open DNA of another organism, insert new gene, make sure it works
- Restriction enzymes cut open DNA-leave unpaired bases-act as "sticky ends"
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