Chromatography

Mind map on chromatography :)

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  • Chromotography
    • Adsorption Chromatography
      • Stationary phase is solid e.g Alumina
      • Moving phase is lquid or gas
  • Chromotogrpahy is a technique for seperating the compounds of a mixture on the basis of differences in their affinity for a stationary phase and a moving phase
  • Partition Chromatography
    • Stationary phase is a non volatile liquid held on a surface inert
    • Chromotography
      • Adsorption Chromatography
        • Stationary phase is solid e.g Alumina
        • Moving phase is lquid or gas
  • Seperation depends on the balance between solute solubility in the moving phase and retention in the stationary phase
    • Partition Chromatography
      • Stationary phase is a non volatile liquid held on a surface inert
  • Paper (Partition Chromatogrpahy)
    • Stationary phase is water adsorbed onto cellulose fibres of paper
  • Spots visible by UV light
    • Thin layer Chromatography
      • Concentrated solution is applied at base rises by capillary action
      • Advantages: quick, efficent, reproducible
  • Adsorption Chromatography
    • Column Chromatography
      • Alumina or Silica gel as stationary phase
      • most strongly absorbed take the longest to flow through the column, the most polar
        • Speed increases by increase pressure
  • Then seperatedin batches and analysed
    • most strongly absorbed take the longest to flow through the column, the most polar
      • Speed increases by increase pressure
  • A detector lined to a recorder produces a chromatogram
    • Each will have a retention time therefore can be compared with known samples
    • Mass spectrometer can be used

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