Types of Chromatography

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  • Created by: Taehgan
  • Created on: 08-03-18 12:17
What is thin layer chromatography used for?
To separate stuff in a mixture
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What is the mobile phase of TLC?
Liquid solvent (e.g. ethanol)
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What is the stationary phase of TLC?
Thin layer of silica or alumina fixed to a glass or metal plate?
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Identify two ways to reveal colourless chemicals.
1) Add flourescent dyes to silica - UV light 2) Iodine vapour
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How do you calculate the Rf value?
Distance traveled by spot / Distance traveled by solvent
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What is column chromatography mostly used for?
Purifying an organic product by separating them from unreacted chemicals or side products
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What is the stationary phase of column chromatography?
A glass column packed with a solid, absorbent material e.g. aluminium oxide coated with water - a slurry
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What is the mobile phase of column chromatography?
A solvent
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How are samples identified in column chromatography?
By the time they take to pass through the column
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What is gas chromatography?
Separation of a mixture of volatile liquids
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What is the stationary phase of gas chromatography?
Solid or a solid coated by a viscous liquid (oil) packed into a long column (coiled and built into an oven)
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What is the mobile phase of gas chromatography?
Unreactive carrier gas e.g. nitrogen
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What is retention time dependent upon in gas chromatography?
1) Time spent moving with the carrier gas 2) Time spent absorbed to the viscous liquid
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What does GC-MS stand for?
Gas chromatography and mass spectroscopy
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Other cards in this set

Card 2

Front

What is the mobile phase of TLC?

Back

Liquid solvent (e.g. ethanol)

Card 3

Front

What is the stationary phase of TLC?

Back

Preview of the front of card 3

Card 4

Front

Identify two ways to reveal colourless chemicals.

Back

Preview of the front of card 4

Card 5

Front

How do you calculate the Rf value?

Back

Preview of the front of card 5
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