Biological tests - biochemistry
- Created by: HeHeHe24
- Created on: 06-12-19 16:36
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- Biological tests
- Iodine
- few drops into sample
- blue black
- starch present
- Emulsion test
- sample mixed with ethanol and water
- cloudy white emulsion
- lipids present
- Reducing sugar (glucose)
- Benedicts test
- add Benedict's reagent
- heat in a water bath
- green - low conc.
- Colour change
- red - high conc.
- Colour change
- Non reducing sugar (sucrose)
- warm with dilute HCl
- neutralises
- add equal volume of Benedict's reagent
- warm in a water bath
- colour change
- green - low conc.
- red - high conc.
- warm with dilute HCl
- Biruet test
- tests for peptide bonds
- mix with 1% NaOH and 1% CuSO4
- violet coloured complex forms with Cu2+
- due to alkaline conditions
- Calorimetry
- measures transmission/absorption of light in a coloured solution
- more conc = less light transmitted
- plot a calibration curve
- absorbance against conc.
- find conc. of an unknown solution
- Chromatography
- separates mixture into its constituents
- stationary phase
- TLC plate or chromatography paper
- Mobile phase
- solvent
- movement depends on polarity and solubility
- highly polar adsorbs to surface
- moves slower & travels less
- due to exposed OH groups being able to form Hydrogen bonds
- identify
- calculate Rf values and compare to known
- highly polar adsorbs to surface
- solutions that make spot visible
- UV
- chemicals fluoresce
- plate glows except where spots travel
- Ninhydrin
- binds to amino acids
- put in oven so it can bind
- Iodine
- enclose dry plate with a few crystals
- hold to warm
- vapour forms that can bind to molecules in spots
- UV
- Uses
- Monitor reactions
- analyse drugs for purity
- drugs testing
- Iodine
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