Biochemical Tests
- Created by: KayshaN
- Created on: 16-10-17 19:22
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- Biochemical Tests
- Carbohydrates (Sugars)
- Reducing Sugars
- Add Benedict's reagent to a sample and heat it a water bath.
- IF POSITIVE: form coloured precipiatate, the higher the conc the further the colour change. Could weigh precipitate.
- IF POSITIVE: form coloured precipiatate, the higher the conc the further the colour change. Could weigh precipitate.
- Add Benedict's reagent to a sample and heat it a water bath.
- Non-Reducing Sugars
- (IF NEG RS)New Sample: add dilute hydrochloric acid and heat. Then neutralise it with sodium hydrogencarbonate. Then carry out Benedict's Test as b4. If positive will form coloured precipitate, if negative will stay blue.(no sugar0
- Starch
- add iodine dissolved in potassium iodide solution.
- if present, sample changes from browny-orange to blue-black
- Reducing Sugars
- Proteins
- Biuret Test
- 1) test solution needs to be alkaline, so add drops sodium hydroxide solution. 2) add copper(II) sulfate solution.
- If protein present solution turns purple. If not remains blue
- Biuret Test
- Lipids
- Emulsion Test
- Shake solution with ethanol for 1min then pour into water.
- If lipid present, solution will turn milky. If not stay clear
- Emulsion Test
- Carbohydrates (Sugars)
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